Purification of Virus-like Particles from Blight-Affected Citrus Trees

Isolation of virus like particles from fecal material obtained from diarrheal calves

Amplification of Citrus tristeza virus from a cDNA clone and infection of citrus trees.

Isolates of the Closterovirus, Citrus tristeza virus (CTV), are populations of disparate genotypes and defective RNAs developed during long periods of vegetative propagation of citrus trees. Because it has not been possible to obtain pure cultures of the virus, it is not known what components of the population are primarily responsible for induction of diseases. We previously developed an infec...

Citrus Section Phloem Anatomy of Citrus Trees: Healthy vs. Greening-affected

Phloem cells from HLB-affected trees become obstructed with callose and P-protein plugs. The presence of these plugs is believed to hinder the translocation of photoassimilates (nitrogenous and reduced carbon compounds) to the root system. However, even with seemingly collapsed phloem tissue, citrus trees remain viable and produce fruit for some time, suggesting either incomplete plugging of ph...

Avian lymphoid leukosis: Virus-like particles in kidney Cells

Characterization of leaf starch from HLB-affected and unaffected-girdled citrus trees

Starch content in leaves of HLB (Huanglongbing or citrus greening)-affected branches increases sharply compared to those from non-HLB trees. Starch not only over-accumulates in photosynthetic cells, but starch grains become prominent in vascular parenchyma and sieve elements as well. These observations imply strong disturbances in starch metabolism and photoassimilate partitioning in HLB-affect...